Journal: Catalysis Today

Article Title: A modified expression of the major hydrolase activator in Hypocrea jecorina ( Trichoderma reesei ) changes enzymatic catalysis of biopolymer degradation

doi: 10.1016/j.cattod.2010.12.038

Figure Lengend Snippet: Growth and clearing properties of H. jecorina QM9414 and nx7. (A) Growth rates of both strains have been determined by cultivation on agar plates containing the indicated carbon source or biopolymer at 30 °C. Additionally, the rate of clearing of the xylan-containing medium was determined by measuring the clearing zone. Values are means of three biological replicates; standard deviation was below 5%. (B) Pictures of H. jecorina QM9414 and nx7 strains on the plates used for determination of growth rates.

Article Snippet: H. jecorina ( T. reesei ) QM9414 (ATCC 26921) was used as parental strain throughout this study and nx7 strain as a recombinant strain constitutively expressing xyr1 .

Techniques: Standard Deviation

Journal: Catalysis Today

Article Title: A modified expression of the major hydrolase activator in Hypocrea jecorina ( Trichoderma reesei ) changes enzymatic catalysis of biopolymer degradation

doi: 10.1016/j.cattod.2010.12.038

Figure Lengend Snippet: Transcription analysis of genes encoding for enzymes involved in the degradation of (hemi)cellulose in H. jecorina QM9414 and nx7 after 40 h of cultivation on xylan.

Article Snippet: H. jecorina ( T. reesei ) QM9414 (ATCC 26921) was used as parental strain throughout this study and nx7 strain as a recombinant strain constitutively expressing xyr1 .

Techniques:

Journal: Catalysis Today

Article Title: A modified expression of the major hydrolase activator in Hypocrea jecorina ( Trichoderma reesei ) changes enzymatic catalysis of biopolymer degradation

doi: 10.1016/j.cattod.2010.12.038

Figure Lengend Snippet: Analysis of the conversion of biopolymers to mono- and disaccharides via enzymatic catalysis using supernatants from H. jecorina fermentations on birch wood xylan. The biopolymers birch wood xylan (A and D), beech wood xylan (B and E), and M. giganteus (C and F) were incubated with supernatants from H. jecorina nx7 (light grey) and QM9414 (dark grey) for 0, 20, 40, 60, 120, 180 min at 40 °C. Afterwards HPLC-analysis was performed using d -xylose (A–C) and xylobiose (D–F) as standards. Values are means of four replicates (two biological replicates used in duplicates in the enzyme assay); standard deviation was below 5%.

Article Snippet: H. jecorina ( T. reesei ) QM9414 (ATCC 26921) was used as parental strain throughout this study and nx7 strain as a recombinant strain constitutively expressing xyr1 .

Techniques: Incubation, Enzymatic Assay, Standard Deviation

Journal: Catalysis Today

Article Title: A modified expression of the major hydrolase activator in Hypocrea jecorina ( Trichoderma reesei ) changes enzymatic catalysis of biopolymer degradation

doi: 10.1016/j.cattod.2010.12.038

Figure Lengend Snippet: Analysis of the conversion of biopolymers to mono- and disaccharides via enzymatic catalysis using supernatants from H. jecorina fermentations on CMC. The biopolymers birch wood xylan (A and D), beech wood xylan (B and E), and M. giganteus (C and F) were incubated with supernatants from H. jecorina nx7 (light grey) and QM9414 (dark grey) for 0, 20, 40, 60, 120, 180 min at 40 °C. Afterwards HPLC-analysis was performed using d -xylose (A–C) and xylobiose (D–F) as standards. Values are means of four replicates (two biological replicates used in duplicates in the enzyme assay); standard deviation was below 5%.

Article Snippet: H. jecorina ( T. reesei ) QM9414 (ATCC 26921) was used as parental strain throughout this study and nx7 strain as a recombinant strain constitutively expressing xyr1 .

Techniques: Incubation, Enzymatic Assay, Standard Deviation

Journal: Catalysis Today

Article Title: A modified expression of the major hydrolase activator in Hypocrea jecorina ( Trichoderma reesei ) changes enzymatic catalysis of biopolymer degradation

doi: 10.1016/j.cattod.2010.12.038

Figure Lengend Snippet: Analysis of the conversion of crystalline cellulose to its monosaccharide via enzymatic catalysis using supernatants from different H. jecorina fermentations. The biopolymer was incubated with supernatants from H. jecorina nx7 (light grey) and QM9414 (dark grey) fermentations on birch wood xylan (A) and CMC (B) for 0, 20, 40, 60, 120, 180 min at 40 °C. Afterwards HPLC-analysis was performed using glucose as standard. Values are means of four replicates (two biological replicates used in duplicates in the enzyme assay); standard deviation was below 5%.

Article Snippet: H. jecorina ( T. reesei ) QM9414 (ATCC 26921) was used as parental strain throughout this study and nx7 strain as a recombinant strain constitutively expressing xyr1 .

Techniques: Incubation, Enzymatic Assay, Standard Deviation

Journal: Catalysis Today

Article Title: A modified expression of the major hydrolase activator in Hypocrea jecorina ( Trichoderma reesei ) changes enzymatic catalysis of biopolymer degradation

doi: 10.1016/j.cattod.2010.12.038

Figure Lengend Snippet: Analysis of the conversion of cellulosic biopolymers to its monosaccharide via enzymatic catalysis using supernatants from H. jecorina fermentations on birch wood xylan. The biopolymers CMC (A) and crystalline cellulose (B) were incubated with supernatants from H. jecorina nx7 (light grey) and QM9414 (dark grey) fermentations for 0, 20, 40, 60, 120, 180 min at 40 °C. Afterwards HPLC-analysis was performed using glucose as standard. Values are means of four replicates (two biological replicates used in duplicates in the enzyme assay); standard deviation was below 5%.

Article Snippet: H. jecorina ( T. reesei ) QM9414 (ATCC 26921) was used as parental strain throughout this study and nx7 strain as a recombinant strain constitutively expressing xyr1 .

Techniques: Incubation, Enzymatic Assay, Standard Deviation

Journal: Frontiers in Microbiology

Article Title: CLP1, a Novel Plant Homeo Domain Protein, Participates in Regulating Cellulase Gene Expression in the Filamentous Fungus Trichoderma reesei

doi: 10.3389/fmicb.2019.01700

Figure Lengend Snippet: The effect of the absence of CLP1 on the vegetative growth and conidiation of T. reesei . (A) Colony growth of the Δ clp1 , Re clp1 , and TU6-RP strains on different carbon sources and conidia formation on malt extract. (B) Quantitative determination of the colony diameters as shown in (A) . (C) Biomass accumulation of the TU6-RP and two independent transformants of Δ clp1 cultured with 1% (w/v) glucose as the sole carbon source. (D) Counting of conidia formed by Δ clp1 , Re clp1 , and TU6-RP strains with a hemocytometer after cultivation on malt extract agar for 5 days. Significant difference ( t test, * p < 0.05, ** p < 0.01) was observed in colony diameter and conidia formation between the TU6-RP and the Δ clp1 strains. Values represent the mean of two to three biological replicates. Error bars are the SD from these replicates.

Article Snippet: T. reesei TU-6 (ATCC MYA-256), a uridine auxotroph of T. reesei , was used throughout this work as the parental strain.

Techniques: Cell Culture

Journal: Frontiers in Microbiology

Article Title: CLP1, a Novel Plant Homeo Domain Protein, Participates in Regulating Cellulase Gene Expression in the Filamentous Fungus Trichoderma reesei

doi: 10.3389/fmicb.2019.01700

Figure Lengend Snippet: Subcellular localization of CLP1 and its mutants. (A,B) Subcellular localization of CLP1, the UIM-deleted mutant, and the PHD mutant with two point mutations (C145A and H150A) tagged with GFP at the C-terminus, respectively. Germinated hypha of T. reesei transformants of mCherry - h2b , mCherry - h2b & clp1 - gfp , mCherry - h2b & clp1_ ΔUIM- gfp , and mCherry - h2b & clp1_ PHDM- gfp cultured on 1% (w/v) glucose or Avicel was subject to fluorescence analyses, respectively. (C) CLP1 is recruited to the cellulase gene promoters upon cellulose induction. ChIP analyses of CLP1 occupancy on the cellulase gene promoters of cel7a , cel7b , and the control gene actin in the P tcu1 - clp1 - gfp and TU6-RP strains cultured on 1% (w/v) Avicel or glucose for 24 h. Significant differences ( t test, ** p < 0.01, * p < 0.05) were observed in the occupancy of CLP1 on the cel7a and cel7b promoters between the control TU6-RP and the P tcu1 - clp1 - gfp strain under Avicel-inducing conditions. Values represent the mean of three biological replicates. Error bars are the SD from these replicates.

Article Snippet: T. reesei TU-6 (ATCC MYA-256), a uridine auxotroph of T. reesei , was used throughout this work as the parental strain.

Techniques: Mutagenesis, Cell Culture, Fluorescence, Control